Recruitment of the SWI-SNF chromatin remodeling complex as a mechanism of gene activation by the glucocorticoid receptor tau1 activation domain.
The SWI-SNF complex has been shown to alter nucleosome conformation in an ATP-dependent manner, leading to increased accessibility of nucleosomal DNA to transcription factors. In this study, we show that the SWI-SNF complex can potentiate the activity of the glucocorticoid receptor (GR) through the N-terminal transactivation domain, tau1, in both ... yeast and mammalian cells. GR-tau1 can directly interact with purified SWI-SNF complex, and mutations in tau1 that affect the transactivation activity in vivo also directly affect tau1 interaction with SWI-SNF. Furthermore, the SWI-SNF complex can stimulate tau1-driven transcription from chromatin templates in vitro. Taken together, these results support a model in which the GR can directly recruit the SWI-SNF complex to target promoters during glucocorticoid-dependent gene activation. We also provide evidence that the SWI-SNF and SAGA complexes represent independent pathways of tau1-mediated activation but play overlapping roles that are able to compensate for one another under some conditions.
Mesh Terms:
Amino Acid Sequence, Cell Line, Chromatin, Fungal Proteins, Gene Expression Regulation, Humans, Molecular Sequence Data, Receptors, Glucocorticoid, Saccharomyces cerevisiae, Signal Transduction, Transcription Factors, Transcriptional Activation, Transfection
Amino Acid Sequence, Cell Line, Chromatin, Fungal Proteins, Gene Expression Regulation, Humans, Molecular Sequence Data, Receptors, Glucocorticoid, Saccharomyces cerevisiae, Signal Transduction, Transcription Factors, Transcriptional Activation, Transfection
Mol. Cell. Biol.
Date: Mar. 01, 2000
PubMed ID: 10688647
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