The actin-binding protein Filamin-A interacts with the metabotropic glutamate receptor type 7.
A yeast two-hybrid screen identified Filamin-A as a binding partner of the metabotropic glutamate receptor type 7b (mGluR7b) splice variant. In addition, Filamin-A interacted with mGluR4a, mGluR5a, mGluR5b, mGluR7a and mGluR8a. Domain mapping revealed that alternative splicing of mGluR4, mGluR7 and mGluR8 C-termini regulated the interaction. A conserved tyrosine within ... mGluR C-termini was identified to mediate the binding to Filamin-A. Protein interactions were verified in biochemical assays using recombinant and native proteins. Finally, co-expression of Filamin-A and mGluR7 splice variants was shown in brain regions. These findings suggest that Filamin-A may physically link metabotropic glutamate receptors to the actin cytoskeleton.
Mesh Terms:
Alternative Splicing, Animals, Brain, Cattle, Cell Line, Contractile Proteins, Humans, Mice, Microfilament Proteins, Organ Specificity, Protein Binding, Protein Isoforms, Protein Structure, Tertiary, Rats, Receptors, Metabotropic Glutamate, Recombinant Proteins, Two-Hybrid System Techniques
Alternative Splicing, Animals, Brain, Cattle, Cell Line, Contractile Proteins, Humans, Mice, Microfilament Proteins, Organ Specificity, Protein Binding, Protein Isoforms, Protein Structure, Tertiary, Rats, Receptors, Metabotropic Glutamate, Recombinant Proteins, Two-Hybrid System Techniques
FEBS Lett.
Date: Mar. 13, 2002
PubMed ID: 11943148
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