p27, a novel inhibitor of G1 cyclin-Cdk protein kinase activity, is related to p21.
Using a yeast interaction screen to search for proteins that interact with cyclin D1-Cdk4, we identified a 27 kDa mouse protein related to the p21 cyclin-Cdk inhibitor. p27 interacts strongly with D-type cyclins and Cdk4 in vitro and more weakly with cyclin E and Cdk2. In mouse fibroblasts, p27 is ... associated predominantly with cyclin D1-Cdk4. Recombinant p27 is a potent inhibitor of cyclin D1-Cdk4 and cyclin A-Cdk2 protein kinase activity and a weaker inhibitor of cyclin B1-Cdc2. Overexpression of p27 in Saos-2 cells causes G1 arrest. p27 protein levels do not change as serum-stimulated quiescent mouse fibroblasts progress through the cell cycle. p27 is identical to p27Kip1, a cyclin-Cdk inhibitor present in TGF beta-treated cells. p27 has the hallmarks of a negative regulator of G1 progression and may mediate TGF beta-induced G1 arrest.
Mesh Terms:
3T3 Cells, Amino Acid Sequence, Animals, Base Sequence, CDC2-CDC28 Kinases, Cell Cycle, Cell Cycle Proteins, Cell Line, Cloning, Molecular, Cyclin D1, Cyclin-Dependent Kinase 2, Cyclin-Dependent Kinase 4, Cyclin-Dependent Kinase Inhibitor p27, Cyclin-Dependent Kinases, Cyclins, Fungal Proteins, G1 Phase, Gene Expression Regulation, Mice, Microtubule-Associated Proteins, Molecular Sequence Data, Oncogene Proteins, Open Reading Frames, Protein Kinase Inhibitors, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, RNA, Messenger, Recombinant Fusion Proteins, Sequence Analysis, DNA, Tumor Suppressor Proteins, Yeasts
3T3 Cells, Amino Acid Sequence, Animals, Base Sequence, CDC2-CDC28 Kinases, Cell Cycle, Cell Cycle Proteins, Cell Line, Cloning, Molecular, Cyclin D1, Cyclin-Dependent Kinase 2, Cyclin-Dependent Kinase 4, Cyclin-Dependent Kinase Inhibitor p27, Cyclin-Dependent Kinases, Cyclins, Fungal Proteins, G1 Phase, Gene Expression Regulation, Mice, Microtubule-Associated Proteins, Molecular Sequence Data, Oncogene Proteins, Open Reading Frames, Protein Kinase Inhibitors, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, RNA, Messenger, Recombinant Fusion Proteins, Sequence Analysis, DNA, Tumor Suppressor Proteins, Yeasts
Cell
Date: Jul. 15, 1994
PubMed ID: 8033213
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