Regulation of AMPA receptor GluR1 subunit surface expression by a 4. 1N-linked actin cytoskeletal association.

The synaptic localization, clustering, and immobilization of neurotransmitter receptors and ion channels play important roles in synapse formation and synaptic transmission. Although several proteins have been identified that interact with AMPA receptors and that may regulate their synaptic targeting, little is known about the interaction of AMPA receptors with the ...
cytoskeleton. In studies examining the interaction of the AMPA receptor GluR1 subunit with neuronal proteins, we determined that GluR1 interacts with the 4.1G and 4.1N proteins, homologs of the erythrocyte membrane cytoskeletal protein 4.1. Using the yeast two-hybrid system and a heterologous cell system, we demonstrated that both 4.1G and 4.1N bind to a membrane proximal region of the GluR1 C terminus, and that a region within the C-terminal domain of 4.1G or 4.1N is sufficient to mediate the interaction. We also found that 4.1N can associate with GluR1 in vivo and colocalizes with AMPA receptors at excitatory synapses. Disruption of the interaction of GluR1 with 4.1N or disruption of actin filaments decreased the surface expression of GluR1 in heterologous cells. Moreover, disruption of actin filaments in cultured cortical neurons dramatically reduced the level of surface AMPA receptors. These results suggest that protein 4.1N may link AMPA receptors to the actin cytoskeleton.
Mesh Terms:
Animals, Bicyclo Compounds, Heterocyclic, Binding Sites, COS Cells, Cell Membrane, Cells, Cultured, Cytoskeletal Proteins, Cytoskeleton, Humans, Immunoblotting, Male, Membrane Proteins, Microfilaments, Mutagenesis, Site-Directed, Neuropeptides, Precipitin Tests, Protein Binding, Rats, Rats, Sprague-Dawley, Receptors, AMPA, Synapses, Thiazoles, Thiazolidines, Transfection, Two-Hybrid System Techniques
J. Neurosci.
Date: Nov. 01, 2000
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