The C-terminus of human glutaminase L mediates association with PDZ domain-containing proteins.
The enzyme glutaminase in brain is responsible for the synthesis of neurotransmitter glutamate. We used the two-hybrid genetic selection system in yeast to look for interactors of glutaminase in human brain. We have identified two proteins containing PDZ domains, alpha1-syntrophin and a glutaminase-interacting protein, named GIP, that showed association with ... human glutaminase L, as deduced from specificity test of the two-hybrid system. The complete GIP cDNA clone has 1315 nucleotides with a 372-base open reading frame encoding a 124-amino acids protein. Glutaminase associates with both PDZ proteins through its C-terminal end; mutagenesis of single amino acids revealed the sequence -ESXV as essential for the interaction. These data suggest the possibility that PDZ domain-containing proteins are involved in the regulation of glutaminase in brain.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Binding Sites, Brain, Calcium-Binding Proteins, Glutaminase, Humans, Intracellular Signaling Peptides and Proteins, Isoenzymes, Membrane Proteins, Molecular Sequence Data, Muscle Proteins, Mutation, Neurotransmitter Agents, Protein Binding, Protein Structure, Tertiary, Sequence Alignment, Two-Hybrid System Techniques
Amino Acid Sequence, Base Sequence, Binding Sites, Brain, Calcium-Binding Proteins, Glutaminase, Humans, Intracellular Signaling Peptides and Proteins, Isoenzymes, Membrane Proteins, Molecular Sequence Data, Muscle Proteins, Mutation, Neurotransmitter Agents, Protein Binding, Protein Structure, Tertiary, Sequence Alignment, Two-Hybrid System Techniques
FEBS Lett.
Date: Jan. 19, 2001
PubMed ID: 11163757
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