Activation of heterotrimeric G-protein signaling by a ras-related protein. Implications for signal integration.
Utilizing a functional screen in the yeast Saccharomyces cerevisiae we identified mammalian proteins that activate heterotrimeric G-protein signaling pathways in a receptor-independent fashion. One of the identified activators, termed AGS1 (for activator of G-protein signaling), is a human Ras-related G-protein that defines a distinct subgroup of the Ras superfamily. Expression ... of AGS1 in yeast and in mammalian cells results in specific activation of Galpha(i)/Galpha(o) heterotrimeric signaling pathways. In addition, the in vivo and in vitro properties of AGS1 are consistent with it functioning as a direct guanine nucleotide exchange factor for Galpha(i)/Galpha(o). AGS1 thus presents a unique mechanism for signal integration via heterotrimeric G-protein signaling pathways.
Mesh Terms:
Amino Acid Sequence, DNA, Complementary, GTP-Binding Protein alpha Subunit, Gi2, GTP-Binding Protein alpha Subunits, GTP-Binding Protein alpha Subunits, Gi-Go, GTP-Binding Proteins, Guanine Nucleotide Exchange Factors, Guanosine Triphosphate, Heterotrimeric GTP-Binding Proteins, Humans, Hydrolysis, Molecular Sequence Data, Protein Binding, Proto-Oncogene Proteins, Saccharomyces cerevisiae, Sequence Homology, Amino Acid, Signal Transduction, ras Proteins
Amino Acid Sequence, DNA, Complementary, GTP-Binding Protein alpha Subunit, Gi2, GTP-Binding Protein alpha Subunits, GTP-Binding Protein alpha Subunits, Gi-Go, GTP-Binding Proteins, Guanine Nucleotide Exchange Factors, Guanosine Triphosphate, Heterotrimeric GTP-Binding Proteins, Humans, Hydrolysis, Molecular Sequence Data, Protein Binding, Proto-Oncogene Proteins, Saccharomyces cerevisiae, Sequence Homology, Amino Acid, Signal Transduction, ras Proteins
J. Biol. Chem.
Date: Aug. 04, 2000
PubMed ID: 10840027
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