CELE_Y40B1B.6, Y40B1B.6

spr-5 encodes H3K4me2 demethylase containing amine oxidase domain that functions to mediate chromatin remodeling and transcriptional regulation orthologous to human histone demethylase LSD1 (lysine-specific demethylase 1 (OMIM: 609132)); loss of spr-5 activity suppresses Egl phenotype of mutations in sel-12/presenilin and derepresses expression of hop-1, which encodes the second C. elegans presenilin; in spr-5 mutants lack of demethylase activity results in germline mortality due to epigenetic defect and is associated with defects in oogenesis and spermatogenesis as well late larval growth delay; sterility in spr-5 mutants is due to retention of H3K4me2 in the PGCs; spr-5 mutants exhibit transgene desilencing in the germline, suggesting that all spermatogenesis-expressed genes are coordinately misregulated; in spr-5 there is heritable accumulation of spermatogenesis-enriched expression due to heritable accumulation of H3K4me2; outcrossing at severely sterile generations rescues the sterility defect of spr-5; SPR-5 interacts with SPR-1, the C. elegans ortholog of CoREST;spr-5 is expressed in all stages, but it is enriched in the adult and highly expressed in the gonad.

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