Regulation of Pim-1 by Hsp90.
The protooncogene Pim-1 encodes serine/threonine protein kinases that are involved in cytokine-mediated cell proliferation and in lymphoma- and leukemogenesis. It is largely unknown how Pim-1 executes its biological effects. Here we show that Pim-1 physically interacts with heat shock protein 90 alpha and beta (Hsp90alpha and beta). The Hsp90-specific inhibitor ... geldanamycin (GA) induced a rapid degradation of Pim-1 and reduced its kinase activity. The expression of Hsp90alpha was regulated by a signal from the cytokine receptor gp130, as is Pim-1's expression. These results indicate that Hsp90 is coordinately regulated with Pim-1 and is involved in the stabilization and function of Pim-1.
Mesh Terms:
Amino Acid Sequence, Antigens, CD, Base Sequence, Cell Line, Cytokine Receptor gp130, DNA Primers, DNA-Binding Proteins, Gene Expression Regulation, HSP90 Heat-Shock Proteins, Membrane Glycoproteins, Molecular Sequence Data, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-pim-1, Reverse Transcriptase Polymerase Chain Reaction, STAT3 Transcription Factor, Signal Transduction, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Trans-Activators
Amino Acid Sequence, Antigens, CD, Base Sequence, Cell Line, Cytokine Receptor gp130, DNA Primers, DNA-Binding Proteins, Gene Expression Regulation, HSP90 Heat-Shock Proteins, Membrane Glycoproteins, Molecular Sequence Data, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-pim-1, Reverse Transcriptase Polymerase Chain Reaction, STAT3 Transcription Factor, Signal Transduction, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Trans-Activators
Biochem. Biophys. Res. Commun.
Date: Mar. 02, 2001
PubMed ID: 11237709
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