Heat shock factor-1 and the heat shock cognate 70 protein associate in high molecular weight complexes in the cytoplasm of NIH-3T3 cells.
Interaction of heat shock transcription factor-1 (HSF-1) with the seventy kilodalton heat shock cognate protein (HSC70) was examined in NIH 3T3 cells. HSF-1 was found in the cytoplasm of non-stressed cells associated with HSC70 in large (Mr 400-500,000) complexes. After heat shock, HSF-1 became concentrated in the nucleus in smaller, ... more stable complexes that did not contain HSC70, an indication of significant rearrangement within the complexes. These experiments show a profound effect of heat shock on the structure and stability of HSF-1 complexes during nuclear localization and support the hypothesis that HSC70 binding may control HSF-1 function.
Mesh Terms:
3T3 Cells, Animals, Cell Nucleus, Cytoplasm, DNA-Binding Proteins, HSP70 Heat-Shock Proteins, Hot Temperature, Humans, Mice, Molecular Weight, Protein Binding, Recombinant Proteins, Transcription Factors
3T3 Cells, Animals, Cell Nucleus, Cytoplasm, DNA-Binding Proteins, HSP70 Heat-Shock Proteins, Hot Temperature, Humans, Mice, Molecular Weight, Protein Binding, Recombinant Proteins, Transcription Factors
Biochem. Biophys. Res. Commun.
Date: Aug. 04, 1995
PubMed ID: 7639722
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