CELE_Y47D3A.4, Y47D3A.4

cku-70 encodes an ortholog of human XRCC6 (Ku70; OMIM:152690, antigen of systemic lupus erythematosus); CKU-70 is required for resistance to ionizing radiation (IR) in somatic tissues (such as motor neurons, vulva or uterus) and in endoreduplicating intestinal cells, but not in the germline; CKU-70 is also required for survival of dauer larvae subjected to IR, and for resistance of developing embryos to methyl methane sulfonate; CKU-70, unlike CKU-80, inhibits DAF-16-dependent thermotolerance, and slightly shortens normal lifespan; CKU-70 binds CKU-80 in yeast two-hybrid assays, and requires HIM-10 for fully effective DNA repair in somatic cells; by orthology, CKU-70 is expected to function (as a heterodimer with CKU-80) in nonhomologous end-joining of double-stranded breaks in DNA; mutant cku-70 late-stage embryos or dauer larvae are hypersensitive to radiation-induced DNA damage in somatic cells; after irradiation, cku-70 mutants tend to display various postembryonic phenotypes (such as slow growth, uncoordinated locomotion, impaired egg-laying, or vulval defects) that are enhanced by him-10 mutations, and that are thought to reflect missegregation of fragmented chromosomes; cku-70(RNAi) animals show a minor extension of lifespan in an rrf-3(pk1426) mutant background, independently of the presence or absence of either functional DAF-2 or germline cells, but a reduction of lifespan with a loss of DAF-16 activity.

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