HIP-I: a huntingtin interacting protein isolated by the yeast two-hybrid system.
We report the discovery of the huntingtin interacting protein I (HIP-I) which binds specifically to the N-terminus of human huntingtin, both in the two-hybrid screen and in in vitro binding experiments. For the interaction in vivo, a protein region downstream of the polyglutamine stretch in huntingtin is essential. The HIP1 ... cDNA isolated by the two-hybrid screen encodes a 55 kDa fragment of a novel protein. Using an affinity-purified polyclonal antibody raised against recombinant HIP-I, a protein of 116 kDa was detected in brain extracts by Western blot analysis. The predicted amino acid sequence of the HIP-I fragment exhibits significant similarity to cytoskeleton proteins, suggesting that HIP-I and huntingtin play a functional role in the cell filament networks. The HIP1 gene is ubiquitously expressed in different brain regions at low level. HIP-I is enriched in human brain but can also be detected in other human tissues as well as in mouse brain. HIP-I and huntingtin behave almost identically during subcellular fractionation and both proteins are enriched in the membrane containing fractions.
Mesh Terms:
Amino Acid Sequence, Blotting, Northern, Blotting, Western, Brain Chemistry, Cloning, Molecular, Cytoskeleton, DNA-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Gene Expression, Humans, Huntington Disease, Molecular Sequence Data, Nerve Tissue Proteins, Nuclear Proteins, Peptides, Recombinant Fusion Proteins, Repetitive Sequences, Nucleic Acid, Saccharomyces cerevisiae, Sequence Analysis
Amino Acid Sequence, Blotting, Northern, Blotting, Western, Brain Chemistry, Cloning, Molecular, Cytoskeleton, DNA-Binding Proteins, Electrophoresis, Polyacrylamide Gel, Gene Expression, Humans, Huntington Disease, Molecular Sequence Data, Nerve Tissue Proteins, Nuclear Proteins, Peptides, Recombinant Fusion Proteins, Repetitive Sequences, Nucleic Acid, Saccharomyces cerevisiae, Sequence Analysis
Hum. Mol. Genet.
Date: Mar. 01, 1997
PubMed ID: 9147654
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