Interaction of activated Ras with Raf-1 alone may be sufficient for transformation of rat2 cells.

v-H-ras effector mutants have been assessed for transforming activity and for the ability of the encoded proteins to interact with Raf-1-, B-Raf-, byr2-, ralGDS-, and CDC25-encoded proteins in the yeast two-hybrid system. Transformation was assessed in rat2 cells as well as in a mutant cell line, rv68BUR, that affords a ...
more sensitive transformation assay. Selected mutant Ras proteins were also examined for their ability to interact with an amino-terminal fragment of Raf-1 in vitro. Finally, possible cooperation between different v-H-ras effector mutants and between effector mutants and overexpressed Raf-1 was assessed. Ras transforming activity was shown to correlate best with the ability of the encoded protein to interact with Raf-1. No evidence for cooperation between v-H-ras effector mutants was found. Signaling through the Raf1-MEK-mitogen-activated protein kinase cascade may be the only effector pathway contributing to RAS transformation in these cells.
Mesh Terms:
Animals, Binding Sites, Cell Cycle Proteins, Cell Line, Cell Transformation, Neoplastic, GTP-Binding Proteins, Genes, Tumor Suppressor, Genes, ras, Mutation, Phosphoprotein Phosphatases, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-raf, Rats, Signal Transduction, Transcription Factors, ral Guanine Nucleotide Exchange Factor, rap GTP-Binding Proteins, ras Proteins, ras-GRF1
Mol. Cell. Biol.
Date: Jun. 01, 1997
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