Presenilin 1 protein directly interacts with Bcl-2.
Presenilin proteins are involved in familial Alzheimer's disease, a neurodegenerative disorder characterized by massive death of neurons. We describe a direct interaction between presenilin 1 (PS1) and Bcl-2, a key factor in the regulation of apoptosis, by yeast two-hybrid interaction system, by co-immunoprecipitation, and by cross-linking experiments. Our data show ... that PS1 and Bcl-2 assemble into a macromolecular complex, and that they are released from this complex in response to an apoptotic stimulus induced by staurosporine. The results support the idea of cross-talk between these two proteins during apoptosis.
Mesh Terms:
Alzheimer Disease, Cell Line, Cloning, Molecular, Hela Cells, Humans, In Situ Nick-End Labeling, Lymphocytes, Membrane Proteins, Presenilin-1, Protein Binding, Proto-Oncogene Proteins c-bcl-2, Recombinant Proteins, Reverse Transcriptase Polymerase Chain Reaction, Staurosporine, Tumor Cells, Cultured
Alzheimer Disease, Cell Line, Cloning, Molecular, Hela Cells, Humans, In Situ Nick-End Labeling, Lymphocytes, Membrane Proteins, Presenilin-1, Protein Binding, Proto-Oncogene Proteins c-bcl-2, Recombinant Proteins, Reverse Transcriptase Polymerase Chain Reaction, Staurosporine, Tumor Cells, Cultured
J. Biol. Chem.
Date: Oct. 22, 1999
PubMed ID: 10521466
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