Isolation of human delta-catenin and its binding specificity with presenilin 1.

We screened proteins for interaction with presenilin (PS) 1, and cloned the full-length cDNA of human delta-catenin, which encoded 1225 amino acids. Yeast two-hybrid assay, GST binding assay and immunoprecipitation demonstrated that delta-catenin interacted with a hydrophilic loop region in the endoproteolytic C-terminal fragment of PS1, but not with that ...
of PS-2. These results suggest that PS1 and PS2 partly differ in function. PS1 loop fragment containing the pathogenic mutation retained the binding ability. We also found another armadillo-protein, p0071, interacted with PS1.
Mesh Terms:
Amino Acid Sequence, Animals, Armadillo Domain Proteins, COS Cells, Cell Adhesion Molecules, Cytoskeletal Proteins, DNA, Complementary, Humans, Membrane Proteins, Molecular Sequence Data, Peptide Fragments, Phosphoproteins, Plakophilins, Precipitin Tests, Presenilin-1, Presenilin-2, Substrate Specificity
Neuroreport
Date: Feb. 25, 1999
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