Homer binds a novel proline-rich motif and links group 1 metabotropic glutamate receptors with IP3 receptors.

Group I metabotropic glutamate receptors (mGluRs) activate PI turnover and thereby trigger intracellular calcium release. Previously, we demonstrated that mGluRs form natural complexes with members of a family of Homer-related synaptic proteins. Here, we present evidence that Homer proteins form a physical tether linking mGluRs with the inositol trisphosphate receptors ...
(IP3R). A novel proline-rich "Homer ligand" (PPXXFr) is identified in group 1 mGluRs and IP3R, and these receptors coimmunoprecipitate as a complex with Homer from brain. Expression of the IEG form of Homer, which lacks the ability to cross-link, modulates mGluR-induced intracellular calcium release. These studies identify a novel mechanism in calcium signaling and provide evidence that an IEG, whose expression is driven by synaptic activity, can directly modify a specific synaptic function.
Mesh Terms:
Amino Acid Sequence, Animals, Calcium, Calcium Channels, Carrier Proteins, Dynamins, GTP Phosphohydrolases, Gene Expression, Genes, Immediate-Early, Glutamic Acid, Inositol 1,4,5-Trisphosphate Receptors, Intracellular Membranes, Ligands, Molecular Sequence Data, Neuropeptides, Proline, Rats, Receptors, Cytoplasmic and Nuclear, Receptors, Metabotropic Glutamate, Time Factors
Neuron
Date: Oct. 01, 1998
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