CELE_Y32F6B.3, Y32F6B.3

crp-1 encodes a novel member of the Rho subfamily of Ras small GTP-binding proteins; genetic analyses indicate that CRP-1 functions in apical membrane trafficking events as well as in the DNA remodeling/transcriptional response that promotes adaptation to endoplasmic reticulum (ER) stress as part of the unfolded protein response (UPR); in regulating the UPR, CRP-1 likely functions together with the CDC-48.1 AAA+ ATPase and the HIM-6 RecQ helicase, with which it interacts in vitro; in C. elegans, a CRP-1::GFP fusion protein is first expressed in embryos in pharyngeal, rectal, and intestinal tissues; at later stage CRP-1::GFP expression is also seen in head and tail support cells (socket and sheath cells), the vulval epithelium, and the excretory cell; when expressed in NIH3T3 cells, CRP-1 localizes to the trans-Golgi network and to recycling endosomes and does not appear to affect actin cytoskeleton reorganization; in vitro assays indicate that CRP-1 is deficient in GTP hydrolysis, has a higher affinity for GDP (as opposed to GTP) binding, and exhibits low GDP-to-GTP nucleotide-exchange activity.

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