Virus infection induces the assembly of coordinately activated transcription factors on the IFN-beta enhancer in vivo.
We have identified a virus-activated factor (VAF) that binds to a regulatory element shared by different virus-inducible genes. We provide evidence that VAF contains two members of the interferon regulatory factor (IRF) family of transcriptional activator proteins (IRF-3 and IRF-7), as well as the transcriptional coactivator proteins p300 and CBP. ... Remarkably, VAF, as well as recombinant IRF-3 and IRF-7 proteins, binds very weakly to the interferon-beta (IFN-beta) gene promoter in vitro. However, in virus-infected cells, both proteins are recruited to the endogenous IFN-beta promoter as part of a protein complex that includes ATF-2/c-Jun and NF-kappa B. These observations provide a unique example of the coordinate activation of multiple transcriptional activator proteins and their highly cooperative assembly into a transcriptional enhancer complex in vivo.
Mesh Terms:
Activating Transcription Factor 2, Animals, Base Sequence, CHO Cells, CREB-Binding Protein, Cell Nucleus, Cricetinae, Cyclic AMP Response Element-Binding Protein, DNA, DNA-Binding Proteins, Enhancer Elements, Genetic, Fungal Proteins, Gene Expression Regulation, Viral, Hela Cells, Humans, Interferon Regulatory Factor-3, Interferon Regulatory Factor-7, Interferon-beta, Leucine Zippers, Molecular Sequence Data, NF-kappa B, Nuclear Proteins, Phosphorylation, Recombinant Fusion Proteins, Respirovirus, Respirovirus Infections, Saccharomyces cerevisiae Proteins, Trans-Activators, Transcription Factors, Transcription, Genetic
Activating Transcription Factor 2, Animals, Base Sequence, CHO Cells, CREB-Binding Protein, Cell Nucleus, Cricetinae, Cyclic AMP Response Element-Binding Protein, DNA, DNA-Binding Proteins, Enhancer Elements, Genetic, Fungal Proteins, Gene Expression Regulation, Viral, Hela Cells, Humans, Interferon Regulatory Factor-3, Interferon Regulatory Factor-7, Interferon-beta, Leucine Zippers, Molecular Sequence Data, NF-kappa B, Nuclear Proteins, Phosphorylation, Recombinant Fusion Proteins, Respirovirus, Respirovirus Infections, Saccharomyces cerevisiae Proteins, Trans-Activators, Transcription Factors, Transcription, Genetic
Mol. Cell
Date: Mar. 01, 1998
PubMed ID: 9660935
View in: Pubmed Google Scholar
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