Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways.
A multiprotein complex isolated from murine cells is identified as a counterpart of the yeast Mediator of transcriptional regulation on the basis of the following: homologs of two subunits of yeast Mediator, Srb7 and Med7, copurify with the complex; peptide sequencing reveals, in addition, homologs of the yeast Mediator subunits ... Rgr1 and Med6; as with yeast Mediator, the mouse complex binds to the RNA polymerase II C-terminal domain (CTD) and stimulates phosphorylation of the CTD by TFIIH. Peptide sequencing also identifies a component of mouse Mediator as a relative of Ring-3 protein, a mitogen-activated nuclear protein kinase, raising the possibility of Mediator as an end point of signal transduction pathways.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, DNA Primers, Mediator Complex, Mice, Molecular Sequence Data, Phosphorylation, RNA Polymerase II, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Signal Transduction, Trans-Activators, Transcription Factors
Amino Acid Sequence, Animals, Base Sequence, DNA Primers, Mediator Complex, Mice, Molecular Sequence Data, Phosphorylation, RNA Polymerase II, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, Signal Transduction, Trans-Activators, Transcription Factors
Proc. Natl. Acad. Sci. U.S.A.
Date: Jul. 21, 1998
PubMed ID: 9671713
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