Complex formation of JAK2 with PP2A, P13K, and Yes in response to the hematopoietic cytokine interleukin-11.
Interleukin-11 is a stromal derived cytokine important in hematopoiesis. IL-11 intracellular signaling travels through cytoplasmic kinases of the Janus family. How JAKs accomplish the multiple functions of IL-11 has not been determined and until recently only a few associated downstream proteins have been identified. We present evidence here for the ... IL-11 induced association of PP2A, P13K, and Yes to JAK2. Reciprocal immunoprecipitations support the mutual involvement of these signaling components in IL-11 mediated signal transduction. This novel finding of JAK2/PP2A binding and release may have relevance to many serine/threonine regulated mechanisms such as P13K, Stat, and MAPK activation. These associations support a model of JAK2 as a protein kinase docking protein of IL-11 signal transduction that may be applicable to other gp130 and JAK signal transduction systems.
Mesh Terms:
1-Phosphatidylinositol 3-Kinase, 3T3 Cells, Animals, Electrophoresis, Polyacrylamide Gel, Glutathione Transferase, Immunoblotting, Interleukin-11, Janus Kinase 2, Mice, Phosphoprotein Phosphatases, Phosphotransferases (Alcohol Group Acceptor), Phosphotyrosine, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-yes, Recombinant Proteins, Signal Transduction, src-Family Kinases
1-Phosphatidylinositol 3-Kinase, 3T3 Cells, Animals, Electrophoresis, Polyacrylamide Gel, Glutathione Transferase, Immunoblotting, Interleukin-11, Janus Kinase 2, Mice, Phosphoprotein Phosphatases, Phosphotransferases (Alcohol Group Acceptor), Phosphotyrosine, Protein-Tyrosine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-yes, Recombinant Proteins, Signal Transduction, src-Family Kinases
Biochem. Biophys. Res. Commun.
Date: Jul. 16, 1996
PubMed ID: 8702385
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