Interaction of human Ku70 with TRF2.
Ku, a heterodimer of 70- and 80-kDa subunits, plays a general role in the metabolism of DNA ends in eukaryotic cells, including double-strand DNA break repair, V(D)J recombination, and maintenance of telomeres. We have utilized the yeast two-hybrid system to identify Ku70-interacting proteins other than Ku80. Two reactive clones were ... found to encode the dimerization domain of TRF2, a mammalian telomeric protein that binds to duplex TTAGGG repeats at chromosome ends. This interaction was confirmed using bacterial fusion proteins and co-immunoprecipitations from eukaryotic cells overexpressing TRF2. The transfected TFR2 colocalized with Ku70.
Mesh Terms:
Animals, Antigens, Nuclear, Base Sequence, Binding Sites, COS Cells, DNA Helicases, DNA-Binding Proteins, Dimerization, Fluorescent Antibody Technique, Humans, Nuclear Proteins, Precipitin Tests, Protein Binding, Protein Structure, Tertiary, Recombinant Fusion Proteins, Repetitive Sequences, Amino Acid, Saccharomyces cerevisiae Proteins, Telomere, Telomeric Repeat Binding Protein 2, Transfection, Two-Hybrid System Techniques
Animals, Antigens, Nuclear, Base Sequence, Binding Sites, COS Cells, DNA Helicases, DNA-Binding Proteins, Dimerization, Fluorescent Antibody Technique, Humans, Nuclear Proteins, Precipitin Tests, Protein Binding, Protein Structure, Tertiary, Recombinant Fusion Proteins, Repetitive Sequences, Amino Acid, Saccharomyces cerevisiae Proteins, Telomere, Telomeric Repeat Binding Protein 2, Transfection, Two-Hybrid System Techniques
FEBS Lett.
Date: Sep. 08, 2000
PubMed ID: 10984620
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