Activation of T cell Raf-1 at mitosis requires the protein-tyrosine kinase Lck.
The serine/threonine protein kinase Raf-1 is activated in response to a variety of growth factors in fibroblasts and hematopoietic cells. In T cells, Raf-1 is activated in response to stimulation through the T cell antigen receptor, the interleukin-2 receptor, and by stimulation of protein kinase C. We demonstrate here that ... in T cells, Raf-1 is also activated during mitosis. The mitotic activation of Raf-1 was not observed in the Lck-deficient cell line, J.CaM.1. During mitosis, Raf-1 was found to interact selectively with a mitotic form of Lck that migrated with a reduced electrophoretic mobility on SDS-polyacrylamide gels. We conclude that Raf-1 is activated during mitosis in T cells and that this mitotic activation of Raf-1 is dependent on the presence of Lck.
Mesh Terms:
Animals, Enzyme Activation, Humans, Lymphocyte Specific Protein Tyrosine Kinase p56(lck), Macromolecular Substances, Mice, Mitosis, Phosphorylation, Protein Binding, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-raf, T-Lymphocytes, Tumor Cells, Cultured, src-Family Kinases
Animals, Enzyme Activation, Humans, Lymphocyte Specific Protein Tyrosine Kinase p56(lck), Macromolecular Substances, Mice, Mitosis, Phosphorylation, Protein Binding, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-raf, T-Lymphocytes, Tumor Cells, Cultured, src-Family Kinases
J. Biol. Chem.
Date: Nov. 29, 1996
PubMed ID: 8939988
View in: Pubmed Google Scholar
Download Curated Data For This Publication
4991
Switch View:
- Interactions 1