Sorting of mannose 6-phosphate receptors mediated by the GGAs.
The delivery of soluble hydrolases to lysosomes is mediated by the cation-independent and cation-dependent mannose 6-phosphate receptors. The cytosolic tails of both receptors contain acidic-cluster-dileucine signals that direct sorting from the trans-Golgi network to the endosomal-lysosomal system. We found that these signals bind to the VHS domain of the Golgi-localized, ... gamma-ear-containing, ARF-binding proteins (GGAs). The receptors and the GGAs left the trans-Golgi network on the same tubulo-vesicular carriers. A dominant-negative GGA mutant blocked exit of the receptors from the trans-Golgi network. Thus, the GGAs appear to mediate sorting of the mannose 6-phosphate receptors at the trans-Golgi network.
Mesh Terms:
ADP-Ribosylation Factors, Adaptor Proteins, Vesicular Transport, Amino Acid Motifs, Amino Acid Sequence, Animals, COS Cells, Carrier Proteins, Cations, Cell Line, Clathrin, Dipeptides, Dogs, Humans, Microscopy, Fluorescence, Molecular Sequence Data, Mutation, Protein Sorting Signals, Protein Structure, Tertiary, Protein Transport, Proteins, Receptor, IGF Type 2, Recombinant Fusion Proteins, Transport Vesicles, Two-Hybrid System Techniques, Yeasts, trans-Golgi Network
ADP-Ribosylation Factors, Adaptor Proteins, Vesicular Transport, Amino Acid Motifs, Amino Acid Sequence, Animals, COS Cells, Carrier Proteins, Cations, Cell Line, Clathrin, Dipeptides, Dogs, Humans, Microscopy, Fluorescence, Molecular Sequence Data, Mutation, Protein Sorting Signals, Protein Structure, Tertiary, Protein Transport, Proteins, Receptor, IGF Type 2, Recombinant Fusion Proteins, Transport Vesicles, Two-Hybrid System Techniques, Yeasts, trans-Golgi Network
Science
Date: Jun. 01, 2001
PubMed ID: 11387475
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