The metastasis suppressor candidate nucleotide diphosphate kinase NM23 specifically interacts with members of the ROR/RZR nuclear orphan receptor subfamily.

We have cloned proteins that interact with the nuclear orphan receptor RZR beta using the yeast two-hybrid system. We identified, amongst a number of other genes, the nucleoside diphosphate kinase (NDPK)-2 also known as Nm23-2, c-myc regulatory factor PuF and differentiation inhibitory factor, RZR beta specifically interacts with Nm23-2 but ...
not with the closely related tumor metastasis suppressor candidate gene product Nm23-1. In contrast ROR alpha interacts with both Nm23 proteins. These findings were corroborated by in vitro interaction assays based on GST-pulldown experiments. With-n-myc we propose a candidate gene regulated by ROR alpha/RZR beta and Nm23, based on the finding that the respective DNA binding sites in the first intron are conserved in several mammalian species.
Mesh Terms:
Animals, Base Sequence, Genes, myc, Humans, Mice, Molecular Sequence Data, Monomeric GTP-Binding Proteins, NM23 Nucleoside Diphosphate Kinases, Nucleoside-Diphosphate Kinase, Protein Binding, Rats, Receptors, Cytoplasmic and Nuclear, Sequence Homology, Nucleic Acid, Substrate Specificity, Transcription Factors
Biochem. Biophys. Res. Commun.
Date: Oct. 03, 1996
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