Possible His to Asp phosphorelay signaling in an Arabidopsis two-component system.
We have so far cloned a cDNA encoding a hybrid-type histidine kinase (ATHK1), three cDNAs encoding phosphorelay intermediates (ATHP1-3), and four cDNAs encoding response regulators (ATRR1-4) from Arabidopsis thaliana. To determine which molecules constitute a His to Asp phosphorelay pathway, we examined protein-protein interactions between them using a pairwise yeast ... two-hybrid analysis, as an initial step. We detected a specific interaction between ATHK1 and ATHP1. We further examined protein-protein interactions between ATHP1-3 and other histidine kinases. We detected interactions between ETR1 and all ATHPs, and between CKI1 and ATHP1 or ATHP2. Interestingly, ERS1 could not interact with any ATHPs. We also examined protein-protein interactions between ATHP1-3 and ATRR1-4. The results indicated that ATHP2 could interact with ATRR4, and that ATHP3 could interact with ATRR1 or ATRR4. However, ATHP1 could not interact with any ATRRs. On the basis of these results, we discuss the possible phosphorelay networks in an Arabidopsis two-component system.
Mesh Terms:
Arabidopsis, Aspartic Acid, Binding Sites, DNA, Complementary, Genes, Plant, Histidine, Models, Biological, Mutation, Plant Proteins, Protein Binding, Protein Kinases, Signal Transduction, Two-Hybrid System Techniques
Arabidopsis, Aspartic Acid, Binding Sites, DNA, Complementary, Genes, Plant, Histidine, Models, Biological, Mutation, Plant Proteins, Protein Binding, Protein Kinases, Signal Transduction, Two-Hybrid System Techniques
FEBS Lett.
Date: Aug. 04, 2000
PubMed ID: 10930573
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