Human nectin3/PRR3: a novel member of the PVR/PRR/nectin family that interacts with afadin.

We have isolated nectin3/PRR3, the fourth human member of the nectin/PRR family, also described as the alpha herpes virus receptor family. Nectin/PRR members are adhesion molecules expressed at intercellular junctions. Nectin3/PRR3 is a transmembrane protein, whose extracellular region contains three Ig-like domains (V, C and C) and shares approximately 30% ...
identity with the other members. It is mainly expressed in testis and placental tissues. SDS-PAGE analyses demonstrate that nectin3/PRR3 has a molecular weight of 83kDa. Nectin1/PRR1L and nectin2/PRR2S and L were found to be specifically expressed at the intercellular junctions. This localization is in part due to the interaction of the C-terminal part of these receptors (ended by the consensus sequence A/EXYV) and the PDZ domain of afadin. In this report we demonstrate that the nectin3/PRR3 receptor carries the A/EXYV consensus sequence and interacts in vivo with both long and short isoforms of afadin. These results suggest that the human nectin3/PRR3 is a new afadin-associated molecule.
Mesh Terms:
Amino Acid Sequence, Base Sequence, Blotting, Northern, Cell Adhesion Molecules, Cell Line, Cloning, Molecular, DNA, Complementary, Female, Gene Expression, Humans, K562 Cells, Kinesin, Male, Microfilament Proteins, Molecular Sequence Data, Myosins, Precipitin Tests, Protein Binding, RNA, Sequence Analysis, DNA, Tissue Distribution, Tumor Cells, Cultured, U937 Cells
Gene
Date: Sep. 19, 2000
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