CEO1, a new protein from Arabidopsis thaliana, protects yeast against oxidative damage.
The Saccharomyces cerevisiae strain WYT, deficient in the YAP1 transcription factor, was used in a molecular screen to identify genes from Arabidopsis thaliana that could overcome the oxidative stress-sensitive phenotype of these yeast cells. A cDNA named CEO1 increased the tolerance to oxidative damage caused by tert-butylhydroperoxide of both the ... Yap1(-) mutant and the wild-type yeast. Additionally, in Yap1(-) yeast, CEO1 also induced cross-tolerance to oxidative damage caused by hydrogen peroxide and diamide. CEO1 was assigned as being part of a small gene family that, until now, is exclusively restricted to plants. In Arabidopsis, CEO1 was produced in all organs, especially in roots and stems. By using the yeast two-hybrid system, proteins that specifically interact with CEO1 in yeast were identified, and putative DNA-binding proteins were consistently recovered.
Mesh Terms:
Amino Acid Sequence, Arabidopsis, Arabidopsis Proteins, Cloning, Molecular, DNA-Binding Proteins, Escherichia coli, Fungal Proteins, Molecular Sequence Data, Multigene Family, Oxidative Stress, Plant Proteins, Plant Roots, Plant Stems, Protein Isoforms, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Sequence Homology, Amino Acid, Transcription Factors
Amino Acid Sequence, Arabidopsis, Arabidopsis Proteins, Cloning, Molecular, DNA-Binding Proteins, Escherichia coli, Fungal Proteins, Molecular Sequence Data, Multigene Family, Oxidative Stress, Plant Proteins, Plant Roots, Plant Stems, Protein Isoforms, Recombinant Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Sequence Alignment, Sequence Homology, Amino Acid, Transcription Factors
FEBS Lett.
Date: Sep. 29, 2000
PubMed ID: 11018516
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