Cyclic AMP promotes a peripheral distribution of melanosomes and stimulates melanophilin/Slac2-a and actin association.
Melanosomes are melanin-containing organelles that belong to a recently individualized group of lysosome-related organelles. Recently, numerous reports have dissected the molecular mechanisms that control melanosome transport, but nothing was known about the possible regulation of melanosome distribution by exogenous physiological stimulus. In the present report, we demonstrate that a physiological ... melanocyte-differentiating agent such as alpha-melanocyte-stimulating hormone, through the stimulation of the cAMP pathway, induces a rapid centrifugal transport of melanosomes, leading to their accumulation at the dendrite tips of melanocytes. Interestingly, the small GTP binding proteins of the p21Rho family and one of their effectors, p160 Rho-associated kinase, but not PKA, play a key role in redistribution of melanosomes at the extremities of the dendrites. Further, we have investigated, at the molecular level, the effect of cAMP on the different proteins involved in the control of melanosome transport. We demonstrate that cAMP stimulates the expression of Rab27a and rapidly increases the interaction of the melanophilin/Slac2-a with actin. Thus, we propose that the stimulation of the interaction between melanophilin/Slac2-a and actin would allow the rapid accumulation of melanosomes in the actin-rich region of the dendrite extremities.
Mesh Terms:
Actins, Adaptor Proteins, Signal Transducing, Animals, Carrier Proteins, Cell Line, Cell Line, Tumor, Cell Membrane, Cyclic AMP, Cyclic AMP-Dependent Protein Kinases, Cytoskeleton, Dendrites, Forskolin, Humans, Intracellular Signaling Peptides and Proteins, Melanoma, Experimental, Melanosomes, Mice, Myosin Heavy Chains, Myosin Type V, Protein Binding, Protein-Serine-Threonine Kinases, alpha-MSH, rab GTP-Binding Proteins, rho-Associated Kinases, rhoA GTP-Binding Protein
Actins, Adaptor Proteins, Signal Transducing, Animals, Carrier Proteins, Cell Line, Cell Line, Tumor, Cell Membrane, Cyclic AMP, Cyclic AMP-Dependent Protein Kinases, Cytoskeleton, Dendrites, Forskolin, Humans, Intracellular Signaling Peptides and Proteins, Melanoma, Experimental, Melanosomes, Mice, Myosin Heavy Chains, Myosin Type V, Protein Binding, Protein-Serine-Threonine Kinases, alpha-MSH, rab GTP-Binding Proteins, rho-Associated Kinases, rhoA GTP-Binding Protein
FASEB J.
Date: Jun. 01, 2004
PubMed ID: 15059972
View in: Pubmed Google Scholar
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