Small Maf proteins interact with the human transcription factor TCF11/Nrf1/LCR-F1.

The human TCF11 gene encodes a ubiquitously expressed bZIP transcription factor of the cap n' collar (CNC) domain family. It has a high sequence similarity to the erythroid-specific bZIP factor p45 NF-E2 in the CNC domain, which is involved in DNA binding. LCR-F1, a TCF11 isoform, is a more potent ...
transcriptional activator than p45 NF-E2 in erythroid cells. We show here that the TCF11 protein interacts to form heterodimers with small Maf proteins, previously shown to dimerize with p45 NF-E2, ECH and Fos. Such heterodimerization significantly alters the DNA binding characteristics of TCF11. While TCF11 alone binds in vitro to the tandem NF-E2 site derived from 5' DNase hypersensitive site 2 in the beta-globin locus control region and to the single NF-E2 site in the porphobilinogen deaminase gene promoter, stronger binding is detected in the presence of small Maf proteins. Using antibodies, TCF11 isoforms bound to the single NF-E2 site were detected in K562 erythroid cell nuclear extracts. These findings place TCF11 as a good candidate for the proposed widely expressed factor(s) known to interact with small Maf proteins and bind NF-E2 sites in a sequence-specific manner resembling NF-E2.
Mesh Terms:
Avian Proteins, Binding Sites, DNA, DNA-Binding Proteins, Erythroid-Specific DNA-Binding Factors, Humans, Hydroxymethylbilane Synthase, Immunoassay, Leucine Zippers, MafB Transcription Factor, MafF Transcription Factor, NF-E2 Transcription Factor, NF-E2 Transcription Factor, p45 Subunit, NF-E2-Related Factor 1, Nuclear Proteins, Nuclear Respiratory Factor 1, Nuclear Respiratory Factors, Oncogene Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-maf, Trans-Activators, Transcription Factors
Nucleic Acids Res.
Date: Nov. 01, 1996
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