Lack of binding observed between human alpha-synuclein and Bcl-2 protein family.
alpha-Synuclein is a presynaptic protein of unknown function that has been implicated in the pathogenesis of Parkinson's disease. To gain insight into the function of alpha-synuclein, the present study examined the association between alpha-synuclein and the following Bcl-2 family proteins: Bcl-2; Bcl-XL; Bcl-associated death promoter (BAD); and Bcl-2-associated X-protein. The ... results of a binding assay using gluthathione S-transferase (GST) fusion alpha-synuclein protein and an immunoprecipitation assay revealed that wild-type or mutant (A30P and A53T) alpha-synuclein (approximately 16 kDa) does not bind to any of these members of the Bcl-2 family. Furthermore, no binding was observed between alpha-synuclein and BAD, regardless of the phosphorylation state of the serine residue in BAD. In contrast, alpha-synuclein was observed to bind to synphilin-1. Although alpha-synuclein has been reported to bind to BAD, modification of alpha-synuclein might be required for such binding to occur.
Mesh Terms:
Animals, Apoptosis, Binding Sites, Brain, COS Cells, Calcineurin, Carrier Proteins, Immunosuppressive Agents, Nerve Tissue Proteins, Neurons, Parkinson Disease, Phosphorylation, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcl-2, Synucleins, Tacrolimus, alpha-Synuclein, bcl-2-Associated X Protein, bcl-Associated Death Protein, bcl-X Protein
Animals, Apoptosis, Binding Sites, Brain, COS Cells, Calcineurin, Carrier Proteins, Immunosuppressive Agents, Nerve Tissue Proteins, Neurons, Parkinson Disease, Phosphorylation, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-bcl-2, Synucleins, Tacrolimus, alpha-Synuclein, bcl-2-Associated X Protein, bcl-Associated Death Protein, bcl-X Protein
Neurosci. Lett.
Date: Dec. 01, 2001
PubMed ID: 11742726
View in: Pubmed Google Scholar
Download Curated Data For This Publication
5406
Switch View:
- Interactions 1