Ubiquitin and the SCF(Grr1) ubiquitin ligase complex are involved in the signalling pathway activated by external amino acids in Saccharomyces cerevisiae.
In response to external amino acids, the permease-like sensor Ssy1 of Saccharomyces cerevisiae activates a pathway leading to transcriptional induction of several permease genes including AGP1 and PTR2. We previously reported that AGP1 induction requires Grr1, the F-box protein part of the SCF(Grr1) ubiquitin-ligase complex. We show here that ubiquitin, ... other components of SCF(Grr1) and the ubiquitin-conjugating enzyme Cdc34 are essential for AGP1 and PTR2 induction. This suggests that transduction to these genes of the amino acid signal generated by Ssy1 involves an SCF(Grr1)-catalysed ubiquitination step.
Mesh Terms:
Amino Acid Transport Systems, Amino Acids, Carrier Proteins, Dose-Response Relationship, Drug, F-Box Proteins, Fungal Proteins, Gene Deletion, Genotype, Intracellular Signaling Peptides and Proteins, Ligases, Membrane Proteins, Membrane Transport Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Signal Transduction, Time Factors, Transcription, Genetic, Transduction, Genetic, Ubiquitin-Protein Ligase Complexes, Ubiquitin-Protein Ligases, Ubiquitins, beta-Galactosidase
Amino Acid Transport Systems, Amino Acids, Carrier Proteins, Dose-Response Relationship, Drug, F-Box Proteins, Fungal Proteins, Gene Deletion, Genotype, Intracellular Signaling Peptides and Proteins, Ligases, Membrane Proteins, Membrane Transport Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Signal Transduction, Time Factors, Transcription, Genetic, Transduction, Genetic, Ubiquitin-Protein Ligase Complexes, Ubiquitin-Protein Ligases, Ubiquitins, beta-Galactosidase
FEBS Lett.
Date: May. 11, 2001
PubMed ID: 11356187
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