Communication of the position of exon-exon junctions to the mRNA surveillance machinery by the protein RNPS1.
In mammalian cells, splice junctions play a dual role in mRNA quality control: They mediate selective nuclear export of mature mRNA and they serve as a mark for mRNA surveillance, which subjects aberrant mRNAs with premature termination codons to nonsense-mediated decay (NMD). Here, we demonstrate that the protein RNPS1, a ... component of the postsplicing complex that is deposited 5' to exon-exon junctions, interacts with the evolutionarily conserved human Upf complex, a central component of NMD. Significantly, RNPS1 triggers NMD when tethered to the 3' untranslated region of beta-globin mRNA, demonstrating its role as a subunit of the postsplicing complex directly involved in mRNA surveillance.
Mesh Terms:
3' Untranslated Regions, Animals, Cell Line, DNA-Binding Proteins, Exons, Fungal Proteins, Globins, Hela Cells, Humans, Macromolecular Substances, Mice, Models, Biological, Precipitin Tests, Protein Binding, RNA Helicases, RNA Splicing, RNA, Messenger, RNA-Binding Proteins, Recombinant Fusion Proteins, Ribonucleoproteins, Saccharomyces cerevisiae Proteins, Trans-Activators, Transfection
3' Untranslated Regions, Animals, Cell Line, DNA-Binding Proteins, Exons, Fungal Proteins, Globins, Hela Cells, Humans, Macromolecular Substances, Mice, Models, Biological, Precipitin Tests, Protein Binding, RNA Helicases, RNA Splicing, RNA, Messenger, RNA-Binding Proteins, Recombinant Fusion Proteins, Ribonucleoproteins, Saccharomyces cerevisiae Proteins, Trans-Activators, Transfection
Science
Date: Sep. 07, 2001
PubMed ID: 11546874
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