c-Raf kinase binds to N-terminal domain of c-Myc.
We have demonstrated that the 50 N-terminal amino acids of c-Myc bind a kinase activity, which phosphorylates Myc in vitro predominantly on Thr8. We also have shown that c-Raf, a widely known Ser/Thr kinase, involved in the Ras signaling pathway, binds to the same portion of c-Myc in vitro. In ... addition we were able to precipitate native c-Myc/Raf complex from various cell lysates. Physical interaction of Myc and Raf may potentially be a part of their well-known functional cooperation.
Mesh Terms:
Adenosine Triphosphate, Binding Sites, Cell Line, Glutathione Transferase, HL-60 Cells, Humans, Phosphorylation, Protein Binding, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-myc, Proto-Oncogene Proteins c-raf, Recombinant Fusion Proteins, T-Lymphocytes
Adenosine Triphosphate, Binding Sites, Cell Line, Glutathione Transferase, HL-60 Cells, Humans, Phosphorylation, Protein Binding, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-myc, Proto-Oncogene Proteins c-raf, Recombinant Fusion Proteins, T-Lymphocytes
FEBS Lett.
Date: Sep. 08, 1997
PubMed ID: 9315742
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