Molecular cloning and functional characterization of mouse coactosin-like protein.
Coactosin was first isolated from Dictyostelium discoideum and, as reported, human coactosin-like protein (CLP) was identified in a yeast two-hybrid screen using 5-lipoxygenase (5LO) as a bait. A mouse CLP (mCLP) cDNA clone was identified among EMBL/GenBank EST sequences. The derived amino acid sequence (142 residues) was 95.1% identical with ... human CLP. Here, we also show that mCLP interacts with actin and 5LO in the two-hybrid system. High-speed cosedimentation assays and GST-binding assays confirmed these protein interactions. In chemical cross-linking experiments, one molecule of mCLP was covalently linked to either one subunit of actin or one molecule of 5LO. The mCLP-F-actin and mCLP-5LO associations were pH-insensitive and Ca(2+)-independent. However, association with actin was best observed at low salt concentrations, while association with 5LO was favored by salt, indicating different binding characteristics.
Mesh Terms:
Actins, Animals, Arachidonate 5-Lipoxygenase, Calcium, Cloning, Molecular, DNA, Complementary, Glutathione Transferase, Hydrogen-Ion Concentration, Immunoblotting, Mice, Microfilament Proteins, Molecular Sequence Data, Potassium Chloride, Protein Binding, Recombinant Fusion Proteins, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Two-Hybrid System Techniques
Actins, Animals, Arachidonate 5-Lipoxygenase, Calcium, Cloning, Molecular, DNA, Complementary, Glutathione Transferase, Hydrogen-Ion Concentration, Immunoblotting, Mice, Microfilament Proteins, Molecular Sequence Data, Potassium Chloride, Protein Binding, Recombinant Fusion Proteins, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Two-Hybrid System Techniques
Biochem. Biophys. Res. Commun.
Date: Jan. 18, 2002
PubMed ID: 11785969
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