Characterization of the interaction of Raf-1 with ras p21 or 14-3-3 protein in intact cells.

Several deletion mutants of Raf-1 were expressed with v-ras p21 or 14-3-3 protein in COS-7 cells and Sf9 cells and the interaction of Raf-1 with ras p21 or with 14-3-3 protein in intact cells was examined. Raf(1-135) (residues 1-135) and Raf(1-322) interacted with v-ras p21, but other deletion mutants such ...
as Raf(136-322) or Raf(321-648) did not. Raf(1-322) interacted with 14-3-3 protein much more efficiently than Raf(321-648) did. While Raf(1-135) did not interact with 14-3-3 protein, Raf(136-322) did. These results clearly indicate that Raf-1 simultaneously interacts with both ras p21 and 14-3-3 protein through the distinct binding domains in intact cells.
Mesh Terms:
14-3-3 Proteins, Animals, Binding Sites, Cell Line, Haplorhini, Humans, Oncogene Protein p21(ras), Protein-Serine-Threonine Kinases, Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-raf, Sequence Deletion, Spodoptera, Tyrosine 3-Monooxygenase
FEBS Lett.
Date: Jul. 17, 1995
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