Identification of a protein that binds to the SH3 region of Abl and is similar to Bcr and GAP-rho.

A Src homology 3 (SH3) region is a sequence of approximately 50 amino acids found in many nonreceptor tyrosine kinases and other proteins. Deletion of the SH3 region from the protein encoded by the c-abl proto-oncogene activates the protein's transforming capacity, thereby suggesting the participation of the SH3 region in ...
the negative regulation of transformation. A complementary DNA was isolated that encoded a protein, 3BP-1, to which the SH3 region of Abl bound with high specificity and to which SH3 regions from other proteins bound differentially. The sequence of the 3BP-1 protein is similar to that of a COOH-terminal segment of Bcr and to guanosine triphosphatase-activating protein (GAP)-rho, which suggests that it might have GAP activity for Ras-related proteins. The 3BP-1 protein may therefore be a mediator of SH3 function in transformation inhibition and may link tyrosine kinases to Ras-related proteins.
Mesh Terms:
3T3 Cells, Amino Acid Sequence, Animals, Binding Sites, Chimera, Cloning, Molecular, GTPase-Activating Proteins, Gene Library, Genes, abl, Genes, src, Glutathione Transferase, Mice, Molecular Sequence Data, Oncogene Proteins, Plasmids, Polymerase Chain Reaction, Prosencephalon, Protein-Tyrosine Kinases, Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-abl, Proto-Oncogene Proteins c-bcr, Proto-Oncogene Proteins pp60(c-src), Recombinant Fusion Proteins, Restriction Mapping, Rho Factor, Sequence Homology, Nucleic Acid, ras GTPase-Activating Proteins
Science
Date: Aug. 07, 1992
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