SUR-8, a conserved Ras-binding protein with leucine-rich repeats, positively regulates Ras-mediated signaling in C. elegans.
We describe the identification and characterization of a novel gene, sur-8, that positively regulates Ras-mediated signal transduction during C. elegans vulval development. Reduction of sur-8 function suppresses an activated ras mutation and dramatically enhances phenotypes of mpk-1 MAP kinase and ksr-1 mutations, while increase of sur-8 dosage enhances an activated ... ras mutation. sur-8 appears to act downstream of or in parallel to ras but upstream of raf. sur-8 encodes a conserved protein that is composed predominantly of leucine-rich repeats. The SUR-8 protein interacts directly with Ras but not with the Ras(P34G) mutant protein, suggesting that SUR-8 may mediate its effects through Ras binding. A structural and functional SUR-8 homolog in humans specifically binds K-Ras and N-Ras but not H-Ras in vitro.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Carrier Proteins, Conserved Sequence, Embryonic Induction, Female, Gene Dosage, Genes, Helminth, Leucine, Mammals, Mitogen-Activated Protein Kinase 1, Molecular Sequence Data, Mutation, Phenotype, Protein Binding, Protein Kinases, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins c-raf, Repetitive Sequences, Nucleic Acid, Sequence Homology, Amino Acid, Signal Transduction, Suppression, Genetic, Vulva, ras Proteins
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Carrier Proteins, Conserved Sequence, Embryonic Induction, Female, Gene Dosage, Genes, Helminth, Leucine, Mammals, Mitogen-Activated Protein Kinase 1, Molecular Sequence Data, Mutation, Phenotype, Protein Binding, Protein Kinases, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins c-raf, Repetitive Sequences, Nucleic Acid, Sequence Homology, Amino Acid, Signal Transduction, Suppression, Genetic, Vulva, ras Proteins
Cell
Date: Jul. 10, 1998
PubMed ID: 9674433
View in: Pubmed Google Scholar
Download Curated Data For This Publication
5790
Switch View:
- Interactions 2