SUR-8, a conserved Ras-binding protein with leucine-rich repeats, positively regulates Ras-mediated signaling in C. elegans.

We describe the identification and characterization of a novel gene, sur-8, that positively regulates Ras-mediated signal transduction during C. elegans vulval development. Reduction of sur-8 function suppresses an activated ras mutation and dramatically enhances phenotypes of mpk-1 MAP kinase and ksr-1 mutations, while increase of sur-8 dosage enhances an activated ...
ras mutation. sur-8 appears to act downstream of or in parallel to ras but upstream of raf. sur-8 encodes a conserved protein that is composed predominantly of leucine-rich repeats. The SUR-8 protein interacts directly with Ras but not with the Ras(P34G) mutant protein, suggesting that SUR-8 may mediate its effects through Ras binding. A structural and functional SUR-8 homolog in humans specifically binds K-Ras and N-Ras but not H-Ras in vitro.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Animals, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Carrier Proteins, Conserved Sequence, Embryonic Induction, Female, Gene Dosage, Genes, Helminth, Leucine, Mammals, Mitogen-Activated Protein Kinase 1, Molecular Sequence Data, Mutation, Phenotype, Protein Binding, Protein Kinases, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins c-raf, Repetitive Sequences, Nucleic Acid, Sequence Homology, Amino Acid, Signal Transduction, Suppression, Genetic, Vulva, ras Proteins
Cell
Date: Jul. 10, 1998
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