PIAS3 (protein inhibitor of activated STAT-3) modulates the transcriptional activation mediated by the nuclear receptor coactivator TIF2.
PIAS3, a member of the protein inhibitor of activated STAT family, was found to interact in vivo and in vitro with TIF2, a previously described coactivator for nuclear receptors. The interaction is mediated by two distinct non-contiguous regions of TIF2. We found that TIF2-PIAS3 interaction occurs through a unique domain ... of PIAS3, very rich in acidic residues and conserved throughout the PIAS family. PIAS3 modulates the ability of TIF2 to mediate ligand-enhanced transcription activation positively or negatively, for different steroid receptors. Taken together, our results indicate a potential role of PIAS3 as transcriptional modulator of TIF2-mediated signalling.
Mesh Terms:
Androgens, Animals, Base Sequence, Binding Sites, COS Cells, Carrier Proteins, Cercopithecus aethiops, Cloning, Molecular, DNA Primers, Embryo, Mammalian, Glucocorticoids, Intracellular Signaling Peptides and Proteins, Methionine, Mice, Mutagenesis, Nuclear Proteins, Nuclear Receptor Coactivator 2, Protein Inhibitors of Activated STAT, Receptors, Progesterone, Recombination, Genetic, Saccharomyces cerevisiae, Sequence Deletion, Signal Transduction, Transcription Factors, Transcriptional Activation, Transfection
Androgens, Animals, Base Sequence, Binding Sites, COS Cells, Carrier Proteins, Cercopithecus aethiops, Cloning, Molecular, DNA Primers, Embryo, Mammalian, Glucocorticoids, Intracellular Signaling Peptides and Proteins, Methionine, Mice, Mutagenesis, Nuclear Proteins, Nuclear Receptor Coactivator 2, Protein Inhibitors of Activated STAT, Receptors, Progesterone, Recombination, Genetic, Saccharomyces cerevisiae, Sequence Deletion, Signal Transduction, Transcription Factors, Transcriptional Activation, Transfection
FEBS Lett.
Date: Aug. 28, 2002
PubMed ID: 12208521
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