ERBIN: a basolateral PDZ protein that interacts with the mammalian ERBB2/HER2 receptor.

The ERBB receptors have a crucial role in morphogenesis and oncogenesis. We have identified a new PDZ protein we named ERBIN (ERBB2 interacting protein) that acts as an adaptor for the receptor ERBB2/HER2 in epithelia. ERBIN contains 16 leucine-rich repeats (LRRs) in its amino terminus and a PDZ (PSD-95/DLG/ZO-1) domain ...
at its carboxy terminus, and belongs to a new PDZ protein family. The PDZ domain directly and specifically interacts with ERBB2/HER2. ERBIN and ERBB2/HER2 colocalize to the lateral membrane of human intestinal epithelial cells. The ERBIN-binding site in ERBB2/HER2 has a critical role in restricting this receptor to the basolateral membrane of epithelial cells, as mutation of the ERBIN-binding site leads to the mislocalization of the receptor in these cells. We suggest that ERBIN acts in the localization and signalling of ERBB2/HER2 in epithelia.
Mesh Terms:
Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Animals, Binding Sites, Biological Transport, Caco-2 Cells, Carrier Proteins, Cell Line, Cell Membrane, Cell Polarity, Dogs, Enzyme Activation, Epithelial Cells, Fluorescent Antibody Technique, Humans, Intestines, Kidney, Mice, Molecular Sequence Data, Mutation, Protein Binding, Protein Structure, Tertiary, RNA, Messenger, Receptor, erbB-2, Sequence Alignment, Substrate Specificity, Two-Hybrid System Techniques
Nat. Cell Biol.
Date: Jul. 01, 2000
Download Curated Data For This Publication
5841
Switch View:
  • Interactions 2