Pim-1 kinase and p100 cooperate to enhance c-Myb activity.
The pim-1 oncogene is regulated by hematopoietic cytokine receptors, encodes a serine/threonine protein kinase, and cooperates with c-myc in lymphoid cell transformation. Using a yeast two-hybrid screen, we found that Pim-1 protein binds to p100, a transcriptional coactivator that interacts with the c-Myb transcription factor. Pim-1 phosphorylated p100 in vitro, ... formed a stable complex with p100 in animal cells, and functioned downstream of Ras to stimulate c-Myb transcriptional activity in a p100-dependent manner. Thus, Pim-1 and p100 appear to be components of a novel signal transduction pathway affecting c-Myb activity, linking all three to the cytokine-regulated control of hematopoietic cell growth, differentiation, and apoptosis.
Mesh Terms:
Acetyltransferases, Animals, Base Sequence, Cells, Cultured, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Mice, Molecular Sequence Data, Nuclear Proteins, Promoter Regions, Genetic, Protein-Serine-Threonine Kinases, Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-myb, Proto-Oncogene Proteins c-pim-1, Signal Transduction, Trans-Activators, ras Proteins
Acetyltransferases, Animals, Base Sequence, Cells, Cultured, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Mice, Molecular Sequence Data, Nuclear Proteins, Promoter Regions, Genetic, Protein-Serine-Threonine Kinases, Proteins, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-myb, Proto-Oncogene Proteins c-pim-1, Signal Transduction, Trans-Activators, ras Proteins
Mol. Cell
Date: Oct. 01, 1998
PubMed ID: 9809063
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