Identification of heterochromatin protein 1 (HP1) as a phosphorylation target by Pim-1 kinase and the effect of phosphorylation on the transcriptional repression function of HP1(1).

Pim-1, a protooncogene product, is a serine/threonine kinase and is thought to play a role in signal transduction in blood cells. Few phosphorylated target proteins for Pim-1, however, have been identified. In the present study, two-hybrid screening to clone cDNAs encoding proteins binding to Pim-1 was carried out, and a ...
cDNA for heterochromatin protein 1gamma (HP1gamma) was obtained. Binding assays both in yeast and in vitro pull-down using the purified HP1gamma and Pim-1 expressed in Escherichia coli showed that Pim-1 directly bound to the chromo shadow domain of HP1gamma. HP1gamma was also associated with Pim-1 in human HeLa cells and the serine clusters located at the center of HP1gamma were phosphorylated by Pim-1 in vitro. Furthermore, a transcription repression activity of HP1gamma was further stimulated by the deletion of the serine clusters targeted by Pim-1. These results suggest that Pim-1 affects the structure or silencing of chromatin by phosphorylating HP1.
Mesh Terms:
Amino Acid Sequence, Binding Sites, Cell Line, Chromosomal Proteins, Non-Histone, Gene Expression Regulation, Hela Cells, Humans, Molecular Sequence Data, Phosphorylation, Protein Binding, Protein-Serine-Threonine Kinases, Proto-Oncogene Proteins, Proto-Oncogene Proteins c-pim-1, Recombinant Fusion Proteins, Repressor Proteins, Sequence Deletion, Serine, Transcription, Genetic, Transfection, Two-Hybrid System Techniques
FEBS Lett.
Date: Feb. 04, 2000
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