Identification of HS1 protein as a major substrate of protein-tyrosine kinase(s) upon B-cell antigen receptor-mediated signaling.
Crosslinking of membrane-bound immunoglobulins, which are B-cell antigen receptors, causes proliferation and differentiation of B cells or inhibition of their growth. The receptor-mediated signaling involves tyrosine phosphorylation of cellular proteins and rapid activation of Src-like kinases. The amino acid sequences of five proteolytic peptides of p75, a major substrate of ... protein-tyrosine(s) in the signaling, showed that p75 is the human HS1 gene product. The HS1 gene is expressed specifically in hematopoietic cells and encodes p75HS1, which carries both helix-turn-helix and Src homology 3 motifs. p75HS1 showed rapid tyrosine phosphorylation and association with a Src-like kinase, Lyn, after crosslinking of membrane-bound IgM. Thus, p75HS1 may be an important substrate of Lyn and possibly other protein-tyrosine kinases upon B-cell antigen receptor-mediated signaling.
Mesh Terms:
Amino Acid Sequence, Antibodies, Antibodies, Monoclonal, Antigens, CD45, B-Lymphocytes, Blood Proteins, Electrophoresis, Polyacrylamide Gel, Humans, Immunoblotting, Molecular Sequence Data, Molecular Weight, Peptide Fragments, Phosphoproteins, Phosphotyrosine, Protein-Tyrosine Kinases, Receptors, Antigen, T-Cell, Signal Transduction, Tumor Cells, Cultured, Tyrosine
Amino Acid Sequence, Antibodies, Antibodies, Monoclonal, Antigens, CD45, B-Lymphocytes, Blood Proteins, Electrophoresis, Polyacrylamide Gel, Humans, Immunoblotting, Molecular Sequence Data, Molecular Weight, Peptide Fragments, Phosphoproteins, Phosphotyrosine, Protein-Tyrosine Kinases, Receptors, Antigen, T-Cell, Signal Transduction, Tumor Cells, Cultured, Tyrosine
Proc. Natl. Acad. Sci. U.S.A.
Date: Apr. 15, 1993
PubMed ID: 7682714
View in: Pubmed Google Scholar
Download Curated Data For This Publication
5967
Switch View:
- Interactions 1