The transcription factor Spi-1/PU.1 binds RNA and interferes with the RNA-binding protein p54nrb.

The protooncogene for Spi-1/PU.1 is an Ets-related transcription factor overexpressed during Friend erythroleukemia. The molecular basis by which Spi-1/PU.1 is involved in the erythroleukemic process remains to be elucidated. By using an immobilized protein binding assay, we have identified a 55-kDa protein as a putative partner of Spi-1/PU.1 protein. Microsequence ...
analysis revealed that this 55-kDa protein was p54nrb (nuclear RNA-binding protein, 54 kDa) a RNA-binding protein highly similar to the splicing factor PSF (polypyrimidine tract-binding protein-associated splicing factor). In this paper, we show that Spi-1/PU.1 impedes the binding of p54nrb to RNA and alters the splicing process in vitro. Moreover, we present evidence that the transcriptional factor Spi-1/PU.1, unlike other Ets proteins, is able to bind RNA. Altogether, these results raise the intriguing possibility that the functional interference observed between Spi-1/PU.1 and RNA-binding proteins might represent a novel mechanism in malignant erythropoiesis.
Mesh Terms:
Alternative Splicing, Animals, DNA-Binding Proteins, Erythropoiesis, Friend murine leukemia virus, Globins, Glutathione Transferase, Hela Cells, Humans, Leukemia, Erythroblastic, Acute, Mice, Molecular Weight, Nuclear Matrix-Associated Proteins, Nuclear Proteins, Octamer Transcription Factors, RNA, RNA Splicing, RNA-Binding Proteins, Recombinant Fusion Proteins, Retroviridae Proteins, Oncogenic, Transcription Factors
J. Biol. Chem.
Date: May. 10, 1996
Download Curated Data For This Publication
6035
Switch View:
  • Interactions 3