Ht31: the first protein kinase A anchoring protein to integrate protein kinase A and Rho signaling.

In an attempt to isolate protein kinase A anchoring proteins (AKAPs) involved in vasopressin-mediated water reabsorbtion, the complete sequence of the human AKAP Ht31 was determined and a partial cDNA of its rat orthologue (Rt31) was cloned. The Ht31 cDNA includes the estrogen receptor cofactor Brx and the RhoA GDP/GTP ...
exchange factor proto-lymphoid blast crisis (Lbc) sequences. The Ht31 gene was assigned to chromosome 15 (region q24-q25). It encodes Ht31 and the smaller splice variants Brx and proto-Lbc. A protein of the predicted size of Ht31 (309 kDa) was detected in human mammary carcinoma and HeLa cells. Anti-Ht31/Rt31 antibodies immunoprecipitated RhoA from primary cultured rat renal inner medullary collecting duct cells, indicating an interaction between the AKAP and RhoA in vivo. These results suggest that Ht31/Rt31 represent a new type of AKAP, containing both an anchoring and a catalytic domain, which appears to be capable of modulating the activity of an interacting partner. Ht31/Rt31 have the potential to integrate Rho and protein kinase A signaling pathways, and thus, are prime candidates to regulate vasopressin-mediated water reabsorbtion.
Mesh Terms:
A Kinase Anchor Proteins, Adaptor Proteins, Signal Transducing, Amino Acid Sequence, Animals, Cells, Cultured, Chromosomes, Human, Pair 15, Cloning, Molecular, Cyclic AMP-Dependent Protein Kinases, Guanine Nucleotide Exchange Factors, Hela Cells, Humans, Kidney Medulla, Molecular Sequence Data, Oncogene Proteins, Proto-Oncogene Proteins, Rats, Signal Transduction, rhoA GTP-Binding Protein
FEBS Lett.
Date: Nov. 02, 2001
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