TAP/NXF1, the primary mRNA export receptor, specifically interacts with a neuronal RNA-binding protein HuD.
Hu proteins are RNA-binding proteins that are implicated in the control of stabilization, nuclear export, and/or translation of specific mRNAs with AU-rich elements (AREs) in the 3'-untranslated region. Three neuron-specific Hu proteins (HuD, HuB, and HuC), but not a ubiquitously expressed Hu protein HuR, have an activity to induce neurite ... outgrowth when they are overexpressed in a rat neuronal cell line PC12. Here we show that TAP/NXF1, the primary export receptor for the bulk mRNA, is a specific binding partner for HuD. In vitro binding experiments using recombinant proteins revealed that the interaction between TAP and HuD is direct and that HuD can form a ternary complex together with both TAP and RNA. Interestingly, HuR does not interact with TAP. These results suggest that HuD acts as a novel adaptor protein to recruit TAP for efficient export of ARE-containing mRNAs in neuronal cells.
Mesh Terms:
Animals, Binding Sites, Hela Cells, Hu Paraneoplastic Encephalomyelitis Antigens, Humans, Nerve Tissue Proteins, Neurites, Neurons, Nucleocytoplasmic Transport Proteins, PC12 Cells, Protein Binding, Protein Structure, Tertiary, RNA Transport, RNA, Messenger, RNA-Binding Proteins, Rats, Sequence Deletion
Animals, Binding Sites, Hela Cells, Hu Paraneoplastic Encephalomyelitis Antigens, Humans, Nerve Tissue Proteins, Neurites, Neurons, Nucleocytoplasmic Transport Proteins, PC12 Cells, Protein Binding, Protein Structure, Tertiary, RNA Transport, RNA, Messenger, RNA-Binding Proteins, Rats, Sequence Deletion
Biochem. Biophys. Res. Commun.
Date: Aug. 20, 2004
PubMed ID: 15358174
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