Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors.
To identify the proteins which may modulate the functions of peroxisome proliferator-activated receptor (PPAR), a rat liver cDNA library was screened by a yeast two-hybrid system, using the mouse PPARalpha as a bait. A protein named nuclear receptor binding factor-1 (NRBF-1) was identified, which interacts not only with PPARalpha, but ... also with various nuclear hormone receptors in the presence of the respective ligands. Both the hinge and ligand-binding domains of PPARalpha are required for the interaction. NRBF-1 seems to be translocated to the nucleus by a piggyback mechanism, together with PPARalpha. NRBF-1 has a significant homology to the yeast protein MRF1, a putative transcription factor regulating the expression of mitochondrial respiratory proteins. NRBF-1 might be another type of nuclear receptor co-operator.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Biological Transport, Carrier Proteins, Cell Nucleus, Cloning, Molecular, DNA, Complementary, DNA-Binding Proteins, Intracellular Signaling Peptides and Proteins, Molecular Sequence Data, Rats, Receptors, Cytoplasmic and Nuclear, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Transcription Factors
Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Biological Transport, Carrier Proteins, Cell Nucleus, Cloning, Molecular, DNA, Complementary, DNA-Binding Proteins, Intracellular Signaling Peptides and Proteins, Molecular Sequence Data, Rats, Receptors, Cytoplasmic and Nuclear, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Transcription Factors
Gene
Date: Oct. 23, 1998
PubMed ID: 9795230
View in: Pubmed Google Scholar
Download Curated Data For This Publication
6204
Switch View:
- Interactions 6