Nuclear receptor binding factor-1 (NRBF-1), a protein interacting with a wide spectrum of nuclear hormone receptors.

To identify the proteins which may modulate the functions of peroxisome proliferator-activated receptor (PPAR), a rat liver cDNA library was screened by a yeast two-hybrid system, using the mouse PPARalpha as a bait. A protein named nuclear receptor binding factor-1 (NRBF-1) was identified, which interacts not only with PPARalpha, but ...
also with various nuclear hormone receptors in the presence of the respective ligands. Both the hinge and ligand-binding domains of PPARalpha are required for the interaction. NRBF-1 seems to be translocated to the nucleus by a piggyback mechanism, together with PPARalpha. NRBF-1 has a significant homology to the yeast protein MRF1, a putative transcription factor regulating the expression of mitochondrial respiratory proteins. NRBF-1 might be another type of nuclear receptor co-operator.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Binding Sites, Biological Transport, Carrier Proteins, Cell Nucleus, Cloning, Molecular, DNA, Complementary, DNA-Binding Proteins, Intracellular Signaling Peptides and Proteins, Molecular Sequence Data, Rats, Receptors, Cytoplasmic and Nuclear, Sequence Alignment, Sequence Analysis, DNA, Sequence Homology, Amino Acid, Transcription Factors
Date: Oct. 23, 1998
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