Preparation of ubiquitinated substrates by the PY motif-insertion method for monitoring 26S proteasome activity.
For analysis of the mechanism of the 26S proteasome-mediated protein degradation in vitro, the preparation of well-defined substrate, the ubiquitinated proteins, of the 26S proteasome is inevitable. However, no method has been available to ubiquitinate a given protein. Here, we propose a relatively simple method for preparation of the ubiquitinated ... substrates using HECT-type ubiquitin ligase Rsp5, termed the PY motif-insertion method. The principle of this method is that the PY motif, known as the Rsp5-binding motif, is inserted into protein to be ubiquitinated by Rsp5. In this communication, we describe that Sic1 was successfully ubiquitinated by the PY motif-insertion method and demonstrate that Sic1 thus ubiquitinated was degraded by the purified yeast 26S proteasome.
Mesh Terms:
Electrophoresis, Polyacrylamide Gel, Endosomal Sorting Complexes Required for Transport, Proteasome Endopeptidase Complex, Saccharomyces cerevisiae Proteins, Substrate Specificity, Ubiquitin, Ubiquitin-Protein Ligase Complexes
Electrophoresis, Polyacrylamide Gel, Endosomal Sorting Complexes Required for Transport, Proteasome Endopeptidase Complex, Saccharomyces cerevisiae Proteins, Substrate Specificity, Ubiquitin, Ubiquitin-Protein Ligase Complexes
Meth. Enzymol.
Date: Dec. 13, 2005
PubMed ID: 16338358
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