The A-kinase-anchoring protein AKAP95 is a multivalent protein with a key role in chromatin condensation at mitosis.
Protein kinase A (PKA) and the nuclear A-kinase-anchoring protein AKAP95 have previously been shown to localize in separate compartments in interphase but associate at mitosis. We demonstrate here a role for the mitotic AKAP95-PKA complex. In HeLa cells, AKAP95 is associated with the nuclear matrix in interphase and redistributes mostly ... into a chromatin fraction at mitosis. In a cytosolic extract derived from mitotic cells, AKAP95 recruits the RIIalpha regulatory subunit of PKA onto chromatin. Intranuclear immunoblocking of AKAP95 inhibits chromosome condensation at mitosis and in mitotic extract in a PKA-independent manner. Immunodepletion of AKAP95 from the extract or immunoblocking of AKAP95 at metaphase induces premature chromatin decondensation. Condensation is restored in vitro by a recombinant AKAP95 fragment comprising the 306-carboxy-terminal amino acids of the protein. Maintenance of condensed chromatin requires PKA binding to chromatin-associated AKAP95 and cAMP signaling through PKA. Chromatin-associated AKAP95 interacts with Eg7, the human homologue of Xenopus pEg7, a component of the 13S condensin complex. Moreover, immunoblocking nuclear AKAP95 inhibits the recruitment of Eg7 to chromatin in vitro. We propose that AKAP95 is a multivalent molecule that in addition to anchoring a cAMP/PKA-signaling complex onto chromosomes, plays a role in regulating chromosome structure at mitosis.
Mesh Terms:
Antibodies, Cell Cycle, Cell Cycle Proteins, Cell Extracts, Chromatin, Chromosomes, Human, Cyclic AMP, Cyclic AMP-Dependent Protein Kinases, DNA-Binding Proteins, Egg Proteins, Fluorescent Antibody Technique, Hela Cells, Humans, Intracellular Signaling Peptides and Proteins, Mitosis, Nocodazole, Nuclear Matrix, Nuclear Proteins, Peptide Fragments, Precipitin Tests, Protein Binding, Signal Transduction, Xenopus Proteins
Antibodies, Cell Cycle, Cell Cycle Proteins, Cell Extracts, Chromatin, Chromosomes, Human, Cyclic AMP, Cyclic AMP-Dependent Protein Kinases, DNA-Binding Proteins, Egg Proteins, Fluorescent Antibody Technique, Hela Cells, Humans, Intracellular Signaling Peptides and Proteins, Mitosis, Nocodazole, Nuclear Matrix, Nuclear Proteins, Peptide Fragments, Precipitin Tests, Protein Binding, Signal Transduction, Xenopus Proteins
J. Cell Biol.
Date: Dec. 13, 1999
PubMed ID: 10601332
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