Rac3-induced neuritogenesis requires binding to Neurabin I.
Rac3, a neuronal GTP-binding protein of the Rho family, induces neuritogenesis in primary neurons. Using yeast two-hybrid analysis, we show that Neurabin I, the neuronal F-actin binding protein, is a direct Rac3-interacting molecule. Biochemical and light microscopy studies indicate that Neurabin I copartitions and colocalizes with Rac3 at the growth ... cones of neurites, inducing Neurabin I association to the cytoskeleton. Moreover, Neurabin I antisense oligonucleotides abolish Rac3-induced neuritogenesis, which in turn is rescued by exogenous Neurabin I but not by Neurabin I mutant lacking the Rac3-binding domain. These results show that Neurabin I mediates Rac3-induced neuritogenesis, possibly by anchoring Rac3 to growth cone F-actin.
Mesh Terms:
Actins, Animals, Cells, Cultured, Cytoskeleton, Growth Cones, Immunoprecipitation, Mice, Microfilament Proteins, Mutation, Nerve Tissue Proteins, Neurites, Neurons, Nuclear Receptor Coactivator 3, Oligodeoxyribonucleotides, Antisense, Protein Interaction Mapping, Protein Structure, Tertiary, Rats, Transcription Factors, Transcriptional Activation
Actins, Animals, Cells, Cultured, Cytoskeleton, Growth Cones, Immunoprecipitation, Mice, Microfilament Proteins, Mutation, Nerve Tissue Proteins, Neurites, Neurons, Nuclear Receptor Coactivator 3, Oligodeoxyribonucleotides, Antisense, Protein Interaction Mapping, Protein Structure, Tertiary, Rats, Transcription Factors, Transcriptional Activation
Mol. Biol. Cell
Date: May. 01, 2006
PubMed ID: 16525025
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