Requirement of ELC1 for RNA polymerase II polyubiquitylation and degradation in response to DNA damage in Saccharomyces cerevisiae.
Treatment of Saccharomyces cerevisiae and human cells with DNA-damaging agents such as UV light or 4-nitroquinoline-1-oxide induces polyubiquitylation of the largest RNA polymerase II (Pol II) subunit, Rpb1, which results in rapid Pol II degradation by the proteasome. Here we identify a novel role for the yeast Elc1 protein in ... mediating Pol II polyubiquitylation and degradation in DNA-damaged yeast cells and propose the involvement of a ubiquitin ligase, of which Elc1 is a component, in this process. In addition, we present genetic evidence for a possible involvement of Elc1 in Rad7-Rad16-dependent nucleotide excision repair (NER) of lesions from the nontranscribed regions of the genome and suggest a role for Elc1 in increasing the proficiency of repair of nontranscribed DNA, where as a component of the Rad7-Rad16-Elc1 ubiquitin ligase, it would promote the efficient turnover of the NER ensemble from the lesion site in a Rad23-19S proteasomal complex-dependent reaction.
Mesh Terms:
DNA Damage, DNA Repair, DNA-Binding Proteins, Epistasis, Genetic, Genes, Fungal, Lysine, Mutation, Polyubiquitin, Protein Processing, Post-Translational, RNA Polymerase II, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Ultraviolet Rays
DNA Damage, DNA Repair, DNA-Binding Proteins, Epistasis, Genetic, Genes, Fungal, Lysine, Mutation, Polyubiquitin, Protein Processing, Post-Translational, RNA Polymerase II, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins, Transcription Factors, Ultraviolet Rays
Mol. Cell. Biol.
Date: Jun. 01, 2006
PubMed ID: 16705154
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