A TRF1:BRF complex directs Drosophila RNA polymerase III transcription.
It has been generally accepted that the TATA binding protein (TBP) is a universal mediator of transcription by RNA polymerase I, II, and III. Here we report that the TBP-related factor TRF1 rather than TBP is responsible for RNA polymerase III transcription in Drosophila. Immunoprecipitation and in vitro transcription assays ... using immunodepleted extracts supplemented with recombinant proteins reveals that a TRF1:BRF complex is required to reconstitute transcription of tRNA, 5S and U6 RNA genes. In vivo, the majority of TRF1 is complexed with BRF and these two proteins colocalize at many polytene chromosome sites containing RNA pol III genes. These data suggest that in Drosophila, TRF1 rather than TBP forms a complex with BRF that plays a major role in RNA pol III transcription.
Mesh Terms:
Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA, Complementary, DNA-Binding Proteins, Drosophila, Drosophila Proteins, Humans, Insect Proteins, Mice, Molecular Sequence Data, RNA Polymerase III, Rabbits, Recombinant Fusion Proteins, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, TATA Box Binding Protein-Like Proteins, TATA-Binding Protein Associated Factors, TATA-Box Binding Protein, Transcription Factor TFIIIB, Transcription Factors, Transcription, Genetic
Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA, Complementary, DNA-Binding Proteins, Drosophila, Drosophila Proteins, Humans, Insect Proteins, Mice, Molecular Sequence Data, RNA Polymerase III, Rabbits, Recombinant Fusion Proteins, Saccharomyces cerevisiae Proteins, Sequence Homology, Amino Acid, TATA Box Binding Protein-Like Proteins, TATA-Binding Protein Associated Factors, TATA-Box Binding Protein, Transcription Factor TFIIIB, Transcription Factors, Transcription, Genetic
Cell
Date: May. 26, 2000
PubMed ID: 10850489
View in: Pubmed Google Scholar
Download Curated Data For This Publication
68002
Switch View:
- Interactions 2